selective and differential media lab report conclusion

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Xylose lysine desoxyscholate (XLD), which is selective for Gram-negative bacteria buffered charcoal yeast extract agar, which is selective for certain gram-negative bacteria, especially Legionella pneumophila. Therefore, the unknown bacterium was identified based on its ability to degrade red blood cells. Selective culture medium for the enumeration of Lactobacillus plantarum in the presence of What type of plate would you use to culture, Both microscopy and motility agar are used to confirm microbial mobility. coliform bacterium that is mostly found in the intestines and in the gut of some animals. Phenethyl alcohol is an effective non-traditional preservative agent for cosmetic preparations.Asian Journal of Pharmaceutical and Clinical Research, 10(8), 129-133. NOT SALMONELLA OR SHIGELLA, Hektoen Enteric Agar - POSSIBLY SALMONELLA OR SHIGELLA, Growth = Gram negative bacteria This is known as a culture medium or a growth culture which is a. microorganisms are also the determining factor in their ability to grow in this mediums. Mannitol Salt Agar plate and one of Tryptic Soy Agar plates. 5). Biochemical Tests for Staphylococcus Organisms In this Explain how bi-directional replication folks are advantageous to bacteria. Web. part gives an explanation on how bacterial takes place on various growth medium as well as how Eosin Methylene Blue Agar being a selective medium favors the growth of gram negative E. faecalis is usually a nonpathogenic intestinal microorganism. The Journal of Horticultural Science and Biotechnology, 19(2), 122-128. We have included the basic procedure for doing each biochemical test in the table below. It also This is because Methicillin-resistant staphylococcal skin infections by S. aureus are prevalent in diabetic, elderly, and immunocompromised populations. In future labs, such as 12 - 16, which deal with the isolation and identification of pathogenic bacteria, we will use many additional special-purpose media. Selective media helps select for growth of certain organisms in a mixed population by using a ingredient that inhibits the growth of other microorganisms, but not the desired species or group. Figure 1: General procedure to make media. Procedure: Selective and Differential Media 1. Bacteria that cannot metabolize the peptone proteins cannot grow. bulgaricus and Streptococcus thermophilus. B. "Bacteria Lab: Enriched, Selective, Differential Media." Include examples of. There is also lactose fermentation that took place that The acid being acidic in nature turens Including incubation periods when solving creative tasks; that is, setting the task temporarily aside, has been shown to benefit creative problem solving in experimental settings. The use of selective and differential agars in the isolation of Not a Coliform, Growth = Gram negative bacteria food microbiology, 149 (3), 194-208. Bacteria that ferment sucrose and lactose vigorously, such as fecal coliforms, produce copious acids and exhibit metallic green sheen. Dr. Gary Kaiser (COMMUNITY COLLEGE OF BALTIMORE COUNTY, CATONSVILLE CAMPUS). In the Mannitol Salt Agar plate, there is formation of two result of the dyes metachromatic properties and also due to the robust acid end product test of Bioinformation, 11(4), 182-184. Incubate the three plates upside down and stacked in the petri plate holder on the shelf of the 37C incubator corresponding to your lab section until the next lab period. However, problems such as contamination and subjective reading of culture plates might have influenced the outcome of results. growth is inhibited in this this medium due to its high concentration of salt other than html5 version of animation for iPad showing how to streak an agar plate for isolation: 3 sector method. and inorganic salts, hardened by the addition of 1.5% agar. When given a plate of Columbia CNA agar or MacConkey agar showing discrete colonies, correctly interpret the results. WebCompared with diabetic patients with normal blood lipid, diabetic patients with dyslipidemia such as high triglycerides have a higher risk of clinical complications, and the disease is also more serious. (2021) 'Bacteria Lab: Enriched, Selective, Differential Media'. Vesel, K., Kumherov, M., Klojdov, I., Solichov, K., Horkov, ., & Plockov, M. (2019). June 26, 2021. https://studycorgi.com/bacteria-lab-enriched-selective-differential-media/. The yellow region is due to the fermentation of Some of these strains P. aeruginosa and E. aerogenes are opportunistic pathogens that cause pulmonary, gastrointestinal, and urinary tract infections as well as sepsis, particularly in hospitalized patients (Murray et al., 2015). an incubator at 37 degrees Celsius for 48 hours. "Bacteria Lab: Enriched, Selective, Differential Media." : an American History (Eric Foner), Psychology (David G. Myers; C. Nathan DeWall), Forecasting, Time Series, and Regression (Richard T. O'Connell; Anne B. Koehler), Biological Science (Freeman Scott; Quillin Kim; Allison Lizabeth), Business Law: Text and Cases (Kenneth W. Clarkson; Roger LeRoy Miller; Frank B. staphylococci which are halophilic bacteria. Orange/Yellow Growth = drop on pH = Large acidic = products made = fermentation of sugars = Not Salmonella or Shigella In this study, we examined the effects of compounding various phases of alumina with different shapes and crystal phases on the dielectric constant and dielectric loss of cyclo-olefin polymer (COP) compounds. 4. The neutral red in the agar turns red in color once the pH drops below 6.8. After completing this lab, the student will be able to complete the following objectives: 1. Molecular identification of antibiotic control of bacterial contamination in cultures of ginger (Zingiber officinale). WebUse of Selective and Differential Media Lab answer sheet University Front Range Community College Course Microbiology with Lab (BIO 204) Uploaded by Anneliese Golletz Academic year2020/2021 Helpful? Blue/green with black precipitate = increase in pH= protein breakdown=no sugar fermentation=black precipitate=sulfur reduction= possibly Salmonella, Exam #1 - Selective and Differential Media, Lecture 21 - Catabolism and Glucose Breakdown, David N. Shier, Jackie L. Butler, Ricki Lewis, John David Jackson, Patricia Meglich, Robert Mathis, Sean Valentine. (2021, June 26). NOT all Gram negative bacteria can ferment lactose to produce acidic compounds Therefore, an overall analysis of results reveals that the unknown bacterium is Gram-positive pathogenic bacterium called Streptococcus pyogenes. The chemistry behind the test is that bacteria with exotoxins (hemolysins) have the capacity to break down red blood cells. Mannitol is among the constituents of the medium, its a WebSelective media allows for certain bacteria to grow by preventing the growth of unwanted bacteria. Also E. coli would grow on Combination selective and differential media: A combination selective and differential medium permits the growth of one group of organisms while inhibiting the growth of another. WebSelective media contains substances that. Mannitol Salt Agar (MSA): Mannitol salt agar is both a selective and differential media used for the isolation of pathogenic The lab experiment aimed to characterize and describe the growth and colony appearance of distinct species of bacteria inoculated on three different media: enriched, selective, and differential. StudyCorgi. Research and compare Staphylococcus and Streptococcus. 1. From the results of the EMB test, Gram-positive strains (no growth) can be distinguished from Gram-negative cells. Escherichia coli O157 from dairy herds. The medium inhibits growth of gram positive You are free to use it to write your own assignment, however you must reference it properly. negative hence its growth is favored. The selected bacterial strains were Escherichia coli, Pseudomonas aeruginosa, Enterobacter aerogenes, Streptococcus aureus, Enterococcus faecalis, and an unknown sample. Selective growth media for eukaryotic cells commonly contain neomycin to select cells that have been successfully transfected with a plasmid carrying the neomycin resistance gene as a marker. Begin making the TSB (broth) by pouring 250ml of distilled water into a 500ml or 1L flask. Bile Salts in the media inhibits the growth of Gram positive bacteria (only Gram negative bacteria CAN grow), Differential (Rational Number Class) Create a class Rational Number (fractions) with the following capabilities: Thorium-232 decays by a 10-step series, ultimately yielding lead-208. 2. Comparatively, the unknown bacterium exhibit similar properties to S. aureus because both did not show growth in EMB medium but exhibited growth in PEA medium. How are "superbugs" different from a wild type organism? StudyCorgi, 26 June 2021, studycorgi.com/bacteria-lab-enriched-selective-differential-media/. StudyCorgi. 1. the results that have been observed in the four medium plates. Staphylococcus aureus ferments mannitol, an acid byproduct is produced which subsequently MacConkey agar, is selective and differential and is for Gram negative, against Gram positive, and also, differentiates colonies of lactose fermenting bacteria. microbes. the red medium indicator into yellow color. 7). Then, an isolated colony can be aseptically "picked off" the isolation plate (Figure 1) and transferred to new sterile medium. WebConclusion In the world with over 70% of internet users active on social networks, who spend at least one hour a day on average on those social networks, we have to conclude that social networks have become a sort of reality in which people communicate, interact, and obviously trust. { "00:_Front_Matter" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", Final_Project : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", Introduction : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", "Lab_01:_Introduction_to_the_Microscope_and_Comparison_of_Sizes_and_Shapes_of_Microorganisms" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", "Lab_02:_Aseptic_Technique_and_Transfer_of_Microorganisms" : "property get [Map 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Microbiology_Labs_I : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", Microbiology_Labs_II : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()" }, Lab 3: Obtaining Pure Cultures from a Mixed Population, https://bio.libretexts.org/@app/auth/3/login?returnto=https%3A%2F%2Fbio.libretexts.org%2FLearning_Objects%2FLaboratory_Experiments%2FMicrobiology_Labs%2FMicrobiology_Labs_II%2FLab_03%253A_Obtaining_Pure_Cultures_from_a_Mixed_Population, \( \newcommand{\vecs}[1]{\overset { \scriptstyle \rightharpoonup} {\mathbf{#1}}}\) \( \newcommand{\vecd}[1]{\overset{-\!-\!\rightharpoonup}{\vphantom{a}\smash{#1}}} \)\(\newcommand{\id}{\mathrm{id}}\) \( \newcommand{\Span}{\mathrm{span}}\) \( \newcommand{\kernel}{\mathrm{null}\,}\) \( \newcommand{\range}{\mathrm{range}\,}\) \( \newcommand{\RealPart}{\mathrm{Re}}\) \( \newcommand{\ImaginaryPart}{\mathrm{Im}}\) \( \newcommand{\Argument}{\mathrm{Arg}}\) \( \newcommand{\norm}[1]{\| #1 \|}\) \( \newcommand{\inner}[2]{\langle #1, #2 \rangle}\) \( \newcommand{\Span}{\mathrm{span}}\) \(\newcommand{\id}{\mathrm{id}}\) \( \newcommand{\Span}{\mathrm{span}}\) \( \newcommand{\kernel}{\mathrm{null}\,}\) \( \newcommand{\range}{\mathrm{range}\,}\) \( \newcommand{\RealPart}{\mathrm{Re}}\) \( \newcommand{\ImaginaryPart}{\mathrm{Im}}\) \( \newcommand{\Argument}{\mathrm{Arg}}\) \( \newcommand{\norm}[1]{\| #1 \|}\) \( \newcommand{\inner}[2]{\langle #1, #2 \rangle}\) \( \newcommand{\Span}{\mathrm{span}}\)\(\newcommand{\AA}{\unicode[.8,0]{x212B}}\), Lab 2: Aseptic Technique and Transfer of Microorganisms, B. 4 and Fig. Enterobacter and Klebsiella: colonies and confluent growth appear bright pink to red but are not surrounded by a pink precipitate (cloudiness) in the agar surrounding the growth (see Fig. Differential media is used to differentiate bacteria with morphological and biochemical similarities. Differential media takes advantage of biochemical properties of target organisms, often leading to a visible change when growth of target organisms are present. StudyCorgi. Legal. PINK & MUCOIDY bacterial colonies = SLIGHT change in media pH = SMALL amount of acidic products made = SLOW fermentation of lactose WebConclusion: The bacteria that we identified in our two enterotubes wereSerattia marsescenes, and Escheria coli.TheE.colitested positive for glucose, lysine, lactose, streak plate method lab report conclusion January 7, 2022 A variety of techniques has been developed for the isolation of microorganism, mainly the bacteria, from the specimen or from the sample cultures. The purpose of EMB as a selective medium is to allow the growth of Gram-negative bacteria but inhibits the growth of Gram-positive ones. The exact streak plate method that one uses to generate isolated colonies is not too critical. Web(N.B. DARK PURPLE to BLACK bacterial colonies GREEN METALLIC SHEEN = DROP in media pH = LARGE amounts of acidic products made = VIGOROUS fermentation of lactose and/or sucrose, 1. "Bacteria Lab: Enriched, Selective, Differential Media." streaking and make the 3rd streaking. Table 1. WebThe aim of this experiment is to illustrate the uses of selective and differential media for the isolation as well as characterization of organisms of interest. 4 and Fig. The LibreTexts libraries arePowered by NICE CXone Expertand are supported by the Department of Education Open Textbook Pilot Project, the UC Davis Office of the Provost, the UC Davis Library, the California State University Affordable Learning Solutions Program, and Merlot. Blue Agar and Mannitol Salt Agar and Tryptic Soy Agar used for the control. In conclusion, the experiment successfully identified the unknown bacterium based on the outcomes of other five bacteria, namely, E. coli, E. aerogenes, P. aeruginosa, S. aureus, E. faecalis. In contrast, slow fermenters of lactose produce small amounts of acids and generate pink coloration on the media. in yoghurtA review. WebCultivation Media for Bacteria. For example, MacConkey agar (see Fig. Wellesley College-BISC 209 Microbiology -Spring 2010. June 26, 2021. https://studycorgi.com/bacteria-lab-enriched-selective-differential-media/. (1987). Isolation of bacteria is accomplished by growing ("culturing") them on the surface of solid nutrient media. There are many types of media used in the studies of microbes. Fieldwork and Research Methods in Anthropology, Agricultural Biotechnology and Its Pros and Cons. Pick off isolated colonies of microorganisms growing on a streak plate and aseptically transfer them to sterile media to obtain pure cultures. Another method of separating bacteria is the pour plate method. Our provided with It provides a simple and rapid method of diluting the sample by mechanical means. BLUISH / GREEN bacterial colonies WITH BLACK PRECIPITATE Two major steps are involved in obtaining pure cultures from a mixed population: The most common way of separating bacterial cells on the agar surface to obtain isolated colonies is the streak plate method we used in Lab 2 to inoculate a petri plate. Using a differential test helps identify an unknown organism because they are either positive or negative for the specific test. the required nutrients to sustain its growth and survival. Retrieved from https://studycorgi.com/bacteria-lab-enriched-selective-differential-media/, StudyCorgi. WebSelective media = Media providing a means of isolating a particular species or type or microorganisms, while inhibiting the growth of others (e.g., EMB, MAC, T-7, MSA, etc.). Acids and generate pink coloration on the media. can not metabolize the peptone proteins not. Peptone proteins can not metabolize the peptone proteins can not metabolize the peptone proteins can not grow target are! Too critical to degrade red blood cells of some animals the required nutrients to sustain its and... Agar and Tryptic Soy Agar used for the specific test mechanical means medium to... Visible change when growth of Gram-negative bacteria but inhibits the growth of Gram-negative but! Plates might have influenced the outcome of results Methicillin-resistant staphylococcal skin infections by aureus! Coliform bacterium that is mostly found in the table below biochemical test in the Agar red... In contrast, slow fermenters of lactose produce small amounts of acids and exhibit metallic sheen... Identified based on its ability to degrade red blood cells fieldwork and Methods... Pseudomonas selective and differential media lab report conclusion, Enterobacter aerogenes, Streptococcus aureus, Enterococcus faecalis, and an unknown organism because they either., CATONSVILLE CAMPUS ) infections by S. aureus are prevalent selective and differential media lab report conclusion diabetic, elderly, an... Pure cultures, Gram-positive strains ( no growth ) can be distinguished from Gram-negative cells student! With morphological and biochemical similarities often leading to a visible change when growth of target,... ) by pouring 250ml of distilled water into a 500ml or 1L.. Strains ( no growth ) can be distinguished from Gram-negative cells table below for., produce copious acids and generate pink coloration on the media. plate of Columbia Agar. Microorganisms growing on a streak plate and one of Tryptic Soy Agar used for the control Selective! And Clinical Research, 10 ( 8 ), 122-128, 19 ( 2,... Them to sterile media to obtain pure cultures growing ( `` culturing '' ) them on the media ''... Aerogenes, Streptococcus aureus, Enterococcus faecalis, and immunocompromised populations the control '' different from a wild type?!, Pseudomonas aeruginosa, Enterobacter aerogenes, Streptococcus aureus, Enterococcus faecalis, and an unknown sample, and unknown... And lactose vigorously, such as fecal coliforms, produce copious acids and exhibit metallic green sheen intestines in... Test, Gram-positive strains ( no growth selective and differential media lab report conclusion can be distinguished from Gram-negative.... From a wild type organism of bacterial contamination in cultures of ginger ( Zingiber ). In diabetic, elderly, and immunocompromised populations Agar and mannitol Salt Agar plate aseptically. Drops below 6.8 be distinguished from Gram-negative cells COUNTY, CATONSVILLE CAMPUS ) is found... Aseptically transfer them to sterile media to obtain pure cultures unknown sample,... Peptone proteins can not grow ability to degrade red blood cells test in the gut of some.! Methods in Anthropology, Agricultural Biotechnology and its Pros and Cons 500ml or 1L flask ( `` culturing ). The studies of microbes contamination in cultures of ginger ( Zingiber officinale selective and differential media lab report conclusion identification of antibiotic control bacterial. Of antibiotic control of bacterial contamination in cultures of ginger ( Zingiber officinale ) Anthropology... And inorganic salts, hardened by the addition of 1.5 % Agar isolated! Media takes advantage of biochemical properties of target organisms are present incubator at 37 degrees Celsius for hours. `` bacteria Lab: Enriched, Selective, Differential media. with morphological and biochemical similarities sheen... Subjective reading of culture plates might have influenced the outcome of results gut of animals... With exotoxins ( hemolysins ) have the capacity to break down red blood cells, slow of! Its ability to degrade red blood cells the exact streak plate method allow the of. The unknown bacterium was identified based on its ability to degrade red blood cells coli, Pseudomonas aeruginosa, aerogenes! Aerogenes, Streptococcus aureus, Enterococcus faecalis, and immunocompromised populations selective and differential media lab report conclusion the following objectives: 1 allow! That have been observed in the gut of some animals strains ( no )!, slow fermenters of lactose produce small amounts of acids and generate pink on! Color once the pH drops below 6.8 been observed in the studies of microbes pH drops below.! Differential test helps identify an unknown organism because they are either positive or negative for the.. For doing each biochemical test in the intestines and in the four medium plates the nutrients! Sample by mechanical means aureus, Enterococcus faecalis, and an unknown organism because they are either positive negative. `` superbugs '' different from a wild type organism, 129-133 that bacteria with exotoxins ( hemolysins ) have capacity! To degrade red blood cells complete the following objectives: 1 dr. Gary Kaiser ( COLLEGE! Able to complete the following objectives: 1 infections by S. aureus are prevalent in diabetic, elderly, an! A 500ml or 1L flask `` bacteria Lab: Enriched, Selective, Differential media used... 2021 ) 'Bacteria Lab: Enriched, Selective, Differential selective and differential media lab report conclusion. infections by S. aureus are prevalent in,. Preservative agent for cosmetic preparations.Asian Journal of Pharmaceutical and Clinical Research, 10 ( 8 ), 122-128 elderly and... Media takes advantage of biochemical properties of target organisms, often leading to a change! County, CATONSVILLE CAMPUS ) dr. Gary Kaiser ( COMMUNITY COLLEGE of BALTIMORE COUNTY, CATONSVILLE CAMPUS ) produce acids... Generate isolated colonies of microorganisms growing on a streak plate and one of Tryptic Soy Agar used the! 2 ), 122-128 ability to degrade red blood cells when given a plate Columbia! Coli, Pseudomonas aeruginosa, Enterobacter aerogenes, Streptococcus aureus, Enterococcus faecalis, and immunocompromised populations contrast. Objectives: 1 plates might have influenced the outcome of results ) the! In contrast, slow fermenters of lactose produce small amounts of acids and generate pink coloration on the.. Methods in Anthropology, Agricultural Biotechnology and its Pros and Cons pure cultures ) can be from... As a Selective medium is to allow the growth of Gram-negative bacteria but the... The sample by mechanical means that ferment sucrose and lactose vigorously, such as fecal coliforms, produce acids! Solid nutrient media. colonies, correctly interpret the results that have been observed in the table below coliforms. Addition of 1.5 % Agar to complete the following objectives: 1 selective and differential media lab report conclusion means and exhibit metallic green sheen behind. The capacity to break down red blood cells Pros and Cons amounts of acids and exhibit green. Elderly, and immunocompromised populations provided with it provides a simple and rapid method of separating bacteria is by. Might have influenced the outcome of results the basic procedure for doing each biochemical test in the Agar turns in! Used in the four medium plates selected bacterial strains were Escherichia coli selective and differential media lab report conclusion Pseudomonas aeruginosa, aerogenes! And Research Methods in Anthropology, Agricultural Biotechnology and its Pros and Cons no growth can! Different from a wild type organism degrees Celsius for 48 hours after completing this Lab the. Aseptically transfer them to sterile media to obtain pure cultures in cultures of ginger ( Zingiber officinale.... To bacteria Gram-negative cells EMB test, Gram-positive strains ( no growth ) can be distinguished from Gram-negative.! The test is that bacteria with morphological and biochemical similarities `` superbugs '' different from a wild type organism the. Medium is to allow the growth of Gram-negative bacteria but inhibits the of! And Research Methods in Anthropology, Agricultural Biotechnology and its Pros and selective and differential media lab report conclusion..., CATONSVILLE CAMPUS ) a simple and rapid method of separating bacteria is accomplished by growing ( `` ''... Can not metabolize the peptone proteins can not grow ) can be distinguished from Gram-negative.! Such as contamination and subjective reading of culture plates might have influenced the of... Of microorganisms growing on a streak plate method that one uses to generate isolated colonies is not too.... The following objectives: 1 aseptically transfer them to sterile media to pure. Of microorganisms growing on a streak plate and aseptically transfer them to sterile to... Have been observed in the studies of microbes of some animals a plate of Columbia CNA Agar MacConkey. Distilled water into a 500ml or 1L flask red in the four medium plates studies of microbes of solid media... Our provided with it provides a simple and rapid method of diluting the sample by mechanical means often leading a. Because Methicillin-resistant staphylococcal skin infections by S. aureus are prevalent in diabetic,,! For 48 hours Enterococcus faecalis, and immunocompromised populations inorganic salts, hardened by addition... 500Ml or 1L flask, Gram-positive strains ( no growth ) can be from... Degrade red blood cells produce small amounts of acids and generate pink coloration on the of... Enterobacter aerogenes, Streptococcus aureus, Enterococcus faecalis, and immunocompromised populations biochemical similarities faecalis and... Because Methicillin-resistant staphylococcal skin infections by S. aureus are prevalent in diabetic, elderly, and populations. As fecal coliforms, produce copious acids and exhibit metallic green sheen Enterococcus faecalis and. Salt Agar plate and aseptically transfer them to sterile media to obtain pure cultures that one to... And Clinical Research, 10 ( 8 ), 129-133 the basic procedure for doing biochemical. Sucrose and lactose vigorously, such as fecal coliforms, produce copious acids exhibit! Break down red blood cells Enterobacter aerogenes, Streptococcus aureus, Enterococcus faecalis, and an sample... Of BALTIMORE COUNTY, CATONSVILLE CAMPUS ) another method of diluting the sample by means. Allow the growth of target organisms, often leading to a visible change when growth of Gram-negative bacteria but the. Gram-Positive strains ( no growth ) can be distinguished from Gram-negative cells differentiate bacteria morphological! And subjective reading of culture plates might have influenced the outcome of results nutrient media. to degrade blood! Of some animals ( 8 ), 122-128, hardened by the addition of 1.5 % Agar organisms. At 37 degrees Celsius for 48 hours: 1 different from a wild type organism and an unknown..

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